Abstract
Metformin is an oral anti hyperglycemic drug used by the patients of diabetes all over the world. In
present study, Metformin hydrochloride (Glucophage) was used to determine the histological changes in liver of
mice. Twenty mice (4-5 weeks old) were given oral gavages of 0.1 ml of 0.00, 31.25, 125 and 500µg/g B.W
(Complete). of Metformin prepared in distilled water, for 04 weeks daily. After 24 hours of last dose, the animals
were anaesthetized torecoverliver tissues following fixation in Bouin’s fluid and processing for histological
preparation using different grades of alcohol and xylene for dehydration. These tissues were later on infiltrated
with paraffin wax, sectioned to size of 4µm and stained with Hematoxylin and Eosin. The histological analysis
revealed that metformin could cause adverse malformations like pyknosis, necrosis, and steatosis.
SHAGUFTA ANDLEEB, ASMATULLAH, SHABANA SHAUKAT, SIDRA NAWAZ. (2018) Hepatotoxic effects induced by a traditional oral Anti-hyperglycemic Drug in healthy Mice, Biologia – Journal of Biological Society of Pakistan, Volume 64 (I), Volume 64 (I).
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