• SYED SHAHID IMRAN BUKHAR

• IQRA AZAM

• ASIR HASSAN ABBASI

• MEERAB DAUD

• NADEEM SHEIKH

• ANDLEEB BATOOL

• RABIA MAHMOOD

• MARYAM MUKHTAR

• MARYAM MUKHTAR

Abstract

Alloxan monohydrate is an organic compound with heterocyclic skeleton; it has been the routine part of all bakery products such as pizza, biscuits and cakes etc. Swiss albino mice of six weeks were divided into control group and experimental group. Experimental group was administered with Alloxan monohydrate (200 mg/kg) intraperitoneally and then livers were extracted after 3, 6 and 12 hours. All the samples were processed for gel run and thermo cycler program, which revealed alterations in the expression of hepatic mRNA (IL-6) in all loads equally confirmed by the results of housekeeping gene βactin. Gel analysis showed changes in the folds of IL-6 in hepatic mRNA compared to control. These finding led to the conclusion that Alloxan can induce an APR, which is evident by rise in expression of IL6. Hence, extensive use of this food shiner should be avoided. Proposal of the current work was to determine the influence of Alloxan monohydrate on IL-6 gene expression, which is a major inflammatory marker.

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