Abstract
Serum protein profile study is useful to monitor changes in concentration and distribution of proteins
(antigen/antibody). The present study was designed to analyze the biochemical effect of two medicinal plants
i.e., Saussurea lappa and Caesalpinia crista on serum protein profiles of buffaloes, naturally infected with
Fasciola, in comparison with Triclabendazole (an allopathic drug used against fasciolosis). Analysis of
fractionized proteins before and after treatment revealed significant differences in the densities of serum proteins
reflecting the changes at the transcriptional as well as translational level. In densitometric analysis, a total of nine
peaks corresponding to nine different protein fractions i.e., 107, 98, 67, 56, 48, 43, 25, 19 and 15 kDa, were
present before treatment in all infected samples. While in healthy control serum samples only eight peaks were
found, which corresponded to peaks in infected sera except peak of 19kDa. After treatment densities of all
fractions were almost to the level of healthy animal’s sera. The protein fraction (19kDa) was either completely
disappeared or significantly diffused, thereby indicating its association with fasciolosis in buffaloes. The nature of
all the protein fractions observed in present study is needed a detailed investigation to identify them as antigen or
antibody associated with fasciolosis
ASMA WAHEED QURESHI, AKHTAR TANVEER, NADEEM SHEIKH. (2016) Effect of Saussurea lappa (roots) and Caesalpinia crista (seeds), on Serum Protein Profile of Fasciola infected Buffaloes, in comparison with Triclabendazole, Biologia – Journal of Biological Society of Pakistan, Volume 62 (I), Issue 1.
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