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The purpose of the study is to develop an efficient, fast, accurate and precise UV spectrophotometric method by using 0.1N HCl as a solvent, for the validation of levofloxacin in injection form. The λmax for levofloxacin was found to be 293nm using 0.1N HCl as a blank. Calibration curve data proved that the proposed method was linear in the concentration range of 3 to 7μg/mL. The proposed method was validated to determine linearity, precision, accuracy, ruggedness and robustness. The results of above analytical parameters proved that the developed method could be used for the routine analysis of levofloxacin in injection dosage form. The proposed method is free from expensive solvents, chemicals and time consuming steps and can be used in quality assurance laboratories of pharmaceutical industries
MUHAMMAD ASLAM, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD, SAMREEN KOUSAR. (2020) Development of a new method for the validation of levofloxacin by UV-Spectrophotometer , Biologia – Journal of Biological Society of Pakistan, Volume 66(I), Issue 2.
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