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A study on the loss of rice production due to bacterial leaf blight of rice has been reported. A total of 23 bacterial isolates were collected from eight districts of Punjab Pakistan and were characterized on the basis of various morphological and biochemical features. After microscopic study, it was concluded that all Xoo isolates were gram-negative, non endospore-forming bacteria and with encapsulated cells. Growth behavior of all the isolates was checked at two different pH levels i.e., 7.0 and 5.5. The number of bacterial colonies recorded at pH 7.0 was significantly higher than the colonies grown at pH 5.5. Growth of bacteria at two different temperatures i.e. 25 ̊C and 40 ̊C was checked and that it was found that most of the isolates showed maximum growth at 25 ̊C while at 40 ̊C isolates showed poor or no growth. Biochemical characterization of Xoo isolates were conducted which showed positive results for methyl red test and indole test while oxidase test was negative for entire set of collected isolates. The 23 isolates inoculated on test tubes exhibited the blackening of medium confirming to be positive against Hydrogen Sulfide (H2S) test. The two isolates (8a-KSK, 1-S) that showed a different behavior might also help to check the diverse behavior of pathogen and is helpful in detecting the new genetic approaches to develop host plant resistance.There is an alarming situation in these areas and there is a dire need to adopt the management practices to control this disease.
NIDA RAFAQAT, MUHAMMAD TARIQ MANZOOR, MUHAMMAD ASHFAQ, MUHAMMAD ALI, MUHAMMAD SAJJAD, MUHAMMAD SABIR, AYESHA BIBI. (2021) Morphological and Biochemical Characterization of Xanthomonas oryzae pv oryzae isolates from Punjab, Pakistan, Biologia – Journal of Biological Society of Pakistan, Volume 67(I), Issue 1.
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