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Heavy metal accumulation in forage poses a threat of toxic effects on the
cattle. This study includes collection of forage, soil and buffalo blood
samples to analyze heavy metals in them. Forages, soil and buffalo blood
were collected from six sites of sampling. Out of which 5 sites were on the
roadside and one site was away from the road. Grinding of the collected
samples of soil and forage was followed by their acid digestion. Buffalo
blood samples were centrifuged and the blood serum was separated
which was then digested using acids. The atomic absorption
spectrophotometer was then used to analyze the heavy metals in the
digested samples. The results were analyzed statistically. Two-way
ANOVA was used to find out variance and correlation in all samples. The
nickel concentration found in the samples of soil, forage and blood was
highest in the samples collected from site II (Chak 50 SB). The bio
concentration factor found for nickel was highest in the samples of site II.
On the other hand, the bio concentration factor for blood and forage was
highest in the samples collected form site IV (Shaheenabad Road). The
correlation result showed that Ni was positively significant when the soil
and forage was correlated unlike the result of correlation for blood and
forage which was negatively significant. The daily intake of metals and
health risk index was found highest in the samples collected from site IV.
The pollution load index was found highest in the samples collected from
site V (Bhalwal Road).
KAFEEL AHMAD, NIMRA ARSHAD, ZAFAR IQBAL KHAN, KINZA WAJID, IJAZ RASOOL NOORKA, MAHPARA SHEHZADI, HAFSA MEMONA, MADIHA SANA, KHALID NAWAZ, MUDASRA MUNIR, IFRA SALEEM MALIK, TASAWAR ABBAS, ABRAR HUSSAIN, MUHAMMAD SHER, MUHAMMAD FAHAD ULLAH, MUHAMMAD AKRAM QAZI, MUHAMMAD NADEEM, MUHAMMAD RIZWAN QURESHI. (2018) Investigation of Nickel in soil, forages and blood plasma of buffaloes with respect to seasonal variations, Biologia – Journal of Biological Society of Pakistan, Volume 64 (II), Volume 64 (II).
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