Abstract
Background: Adoptability of biotech crops has climbed up dramatically in the world from
1.7 million hectares in its introductory period in 1996 to 170 million hectare in 2012. Area
planted to biotech crop increased to over one-fourth of the world total in 2005-06. The data
collected also demonstrates that developing countries like Pakistan are making tremendous
development in production of Biotech crops especially cotton.
Methodology: Random samples have been collected and received from different cotton
growing areas of Pakistan from 2007 to date for Bt gene confirmation and expression.
Genomic DNA was isolated and analyzed through internal reference primers from SadI gene
of cotton genome. Samples were analyzed by PCR for detection of Bt genes including CEMB
Cry1Ac+2A double Bt gene. ELISA was done for confirmation of Bt protein by using
Envirologix Quantiplate ELISA kit Cry1Ab/Ac Cat # AP003 and Quantiplate ELISA kit
Cry2A Cat # AP005 according to manufacturer instruction.
Results: Development of Bt Cotton in Pakistan is as old as in advanced countries of the
world. Pakistan has become the fourth country of the world for production of indigenous Bt
cotton by utilizing their local cotton varieties for genetic modifications. The support of
various documentary proofs like research articles, patents, projects, Ph. D and M.Phil studies
generated by Centre of Excellence in Molecular Biology (CEMB) greatly strengthen the data
of Bt cotton development in Pakistan. Pakistan Central Cotton Committee (PCCC)declared
locally developed Bt cotton as the best performing of all over Pakistan among other
international product.
Conclusion: Progress made in development of indigenous Bt cotton variety by CEMB
Pakistan and their excellent performance in field determined their increased adoptability ratio
in farmers. The formal approval process which is going to be completed in near future will
open the doors for farmers and breeders to utilize this material for better economy of
Pakistan.
Saira Azam, Tahir Rehman Samiullah, Aneela Yasmeen, Salah ud Din, Adnan Iqbal, Abdul Qayyum Rao, Idrees Ahmad Nasir, Bushra Rashid, Ahmad Ali Shahid, Munir Ahmad, Tayyab Husnain. (2013) Dissemination of Bt cotton in cotton growing belt of Pakistan , Advancements in Life Sciences, Volume 1 , Issue 1.
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