Abstract
Background: An efficient method of artificial mass propagation was optimized for two very
nutritious vegetables of Pakistan, the cabbage and cauliflower.
Methodology: Hypocotyls of germinating of seeds (5-7 days old seedlings) were used as
explants. Murashige and Skoog basal medium was supplemented with different concentrations
of auxin (2,4 Dichlorophenoxy acetic acid) and cytokinin (benzyl amino purine) in
combination to study the callus forming tendency of cabbage and cauliflower; and found better
if used in ratio of 2:1 (at least) respectively.
Results: For evaluation of regeneration potential Kinetin, Zeatin, Gibrellic acid-3, and Indole
acetic acid were found good in combination with benzyl amino purine. Higher (than auxin)
concentration of cytokinin was essential to obtain good regeneration response of callus.
Conclusion: Effectively concluded that these techniques can be used to raise the disease free
stock of cabbage and cauliflower for genetic improvement of in-vitro bulk of varieties on
commercial scale in a very brief time span.
Zahida Qamar, Idrees Ahmad Nasir, Ghulam Zahra Jahangir, Tayyab Husnain. (2013) In-Vitro propagation of hybrid cabbage and cauliflower, Advancements in Life Sciences, Volume 1 , Issue 2 .
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