Abstract
Background: Sacchrum officinarum is acknowledged as a basic source for the production of
sugar in Pakistan and worldwide, one of the major constituents of human diet. The presented
study optimizes a convenient and successful protocol for in-vitro mass production of sugarcane
comprising sixteen cultivars from various sugarcane growing areas all over Pakistan.
Methodology: The source plants were sampled randomly from cane growing areas all over the
country. Apical region from stalks of germinated plants was taken as explant source. The
growth medium used for direct regeneration and multiple shoot formation was same for all
constituents but the hormonal supplementations; it comprised of MS basal medium 4.43g/l (MS
SIGMA, M 5519), 3% w/v sucrose, and phytagel was added in 0.3% w/v for gelling to support
the explant, 0.01mg/l activated charcoal as the carbon source, pH 5.5 to 5.8.
Results: Among various concentrations of BAP used 1.0, 1.5 and 2.0mg/l in growth medium
supported efficient regeneration and plenty of lateral shoots in a minimum time span almost in
all cultivars. For rhizogenesis, 5.0mg/l of IAA was found to be most efficient among four
different concentrations of auxin. Some cultivars have a sufficient endoauxin level and do not
need any supplementation for rooting i.e., basal medium supports root induction. For long term
maintenance of plant stock, MS broth with 1.0mg/l of BAP was found to be most suitable.
Conclusion: Cytokinin concentrations and plant potential play an equal role in direct
regeneration from meristematic tissue.
Ghulam Zahara Jahangir, Idrees Ahmad Nasir, Muhammad Iqbal. (2013) Disease free and rapid mass production of sugarcane cultivars, Advancements in Life Sciences, Volume 1 , Issue 3.
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