Abstract
Background: The importance of cotton crop (Gossypium hirsutum) in textile industry is
based on its fiber quality. A number of fiber-specific genes play important role in the
development of cotton fiber. Previous studies have demonstrated the importance of genes
that are responsible for metabolic functions and their involvement in cotton fiber
development.
Method: This study was focused at successful Agrobacterium mediated stable transformation
of the fiber gene CpTIP1, isolated from the wild plant Calotropis procera, into cotton variety
NIAB-846 for one generation.
Results: Transformation efficiency was calculated to be 1.01% for the target gene. Different
molecular techniques such as PCR were used for confirmation and Real-Time PCR was used
to check the level of quantitative expression of fiber expansin gene in putative transgenic
cotton plants. On the base of molecular analysis, results showed higher expression level of
fiber gene (CpTIP1) in transgenic plants as compared to the control plants.
Conclusion: The results of this study support the idea of improved cotton fiber through
genetic modification especially the cotton fiber strength.
Sidra Akhtar, Ahmad Ali Shahid, Abdul Qayyum Rao, Kamran Shehzad Bajwa, Adnan Muzaffar, Ayesha Latif, Tayyab Husnain. (2013) Genetic effects of Calotropis procera CpTIP1 gene on fiber quality in cotton (Gossypium hirsutum), Advancements in Life Sciences, Volume 1 , Issue 4.
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