Abstract
Calcium-sensing receptor (CaSR) gene polymorphism A986S (rs1801725) is a genetic factor of the
calcium homeostasis and susceptibility of osteoporosis. Although, its role in premenopausal and
postmenopausal women with osteoporosis is yet to be investigated. Therefore, this study was
conducted to assess the CaSR gene polymorphism A986S and evaluate its correlation with
biochemical parameters in premenopausal and postmenopausal Iraqi women with osteoporosis. Blood
samples were obtained from 100 women (53 premenopausal and 47 postmenopausal) diagnosed with
osteoporosis by specialist physicians and 70 healthy women of the same age as the control group.
Serum calcium and phosphorus concentrations were estimated. Genomic DNA was extracted from the
whole blood and used for polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) analysis to detect CaSR A986S polymorphism. The results obtained showed that calcium and
phosphorus levels were significantly lower (p value < 0.01) in the osteoporosis women compared
with healthy groups. Frequencies of T allele and TT genotype were significantly higher (p value <
0.01) in the osteoporosis patients compared with controls, while there were no significant differences
in the frequencies of the patient group. Conversely, there were no differences in the calcium and
phosphorus levels and there was the presence of T allele of the CaSR A986S genotypes. Thus, our
finding revealed that the CaSR polymorphism A986S was one of the genetic susceptibility factors for
the premenopausal and postmenopausal in Iraqi women with osteoporosis and had little effects on
mineral levels.
Adnan F. Al-Azzawie. (2021) Molecular assessment of calcium-sensing receptor gene polymorphism rs1801725 in Iraqi women with osteoporosis, Asian Journal of Agriculture and Biology, Volume-09, Issue-2.
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