Abstract
The objective of the present study to check free-radical-scavenging and hepatoprotective properties of
methanolic extract of Monotheca buxifolia (Falc.) A. DC. aerial parts against isoniazid and rifampicin
induced hepatotoxicity in male rats. Results exhibited that methanolic extract and all three sub-fractions
(hexane, chloroform and ethyl acetate) of M. buxifolia displayed greater free-radical activity, total phenolic
and flavonoid content. However, ethyl-acetate followed by chloroform sub fractions showed the better total
phenolic and total flavonoid content. To assess hepatoprotective activity of methanolic extract of M.
buxifolia, four groups of the rate were made. Group 1 served as a control, group 2 served as hepatotoxic
group (isoniazid and rifampicin orally each 50 mg/kg), group 3 served as standard group received silymarin
(200 mg/kg), and 4 served as extract treatment groups, received 500 mg/kg methanolic extract. The results
suggested that level of liver markers (total bilirubin, alkaline phosphatases, alanine aminotransferases and
aspartate aminotransferases) significantly elevated in group 2. The methanolic extract significantly
prevented isoniazid-rifampicin by decreasing raised level of liver markers of Group 4, and also improved
histopathological profile of liver. The results indicated the protective effect of methanolic extract of M. buxifolia against liver injury, which might be ascribed to presence of high level of phenolics and flavonoid
along with high free-radical activity in the plant extract.
Shabnam Javed, Amna Shoaib, Zaid Mahmood, Saiqa Ishtiaq. (2021) Hepatoprotective Effect of Methanolic Extract of Monotheca buxifolia against Isoniazid and Rifampicin Induced Hepatotoxicity, Asian Journal of Agriculture and Biology, Volume-09, Issue-2.
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