One of the practical applications of Bioinformatics is sequence analysis through sequence DOLJQPHQWRIQHZO\LGHQWL¿HGJHQHVDQGSUHYLRXVO\DYDLODEOHVHTXHQFHVWRGHWHUPLQHWKHVLPLODULW\EHWZHHQ WKHELRORJLFDOVHTXHQFHVDQGHOXFLGDWHWKHIXQFWLRQDOLW\RIJHQHVZLWKFRQ¿GHQFH$OWKRXJKWKHUHDUHPDQ\
softwares available for the biological sequence alignment; in this paper some basic alignment algorithms are discussed and implemented in MATLAB Bioinformatics tool box. Two sequences analyzed by this tool box were muRdr1H and resistance protein of Populus trichocarpa (ACCESSION XP_002329162). muRdr1H is a functionally characterized member of Rdr1 resistance gene family of roses, active against black spot. The VLPXODWHGUHVXOWVKRZVWKDWWKH0$7/$%WRROLVDFRPSUHKHQVLYHWRROIRU¿QGLQJEHVWSRVVLEOHORFDODQG
global sequence alignment. Our proposed work provides useful application of MATLAB which can help in interpretation and visualization of the data in molecular biology