Abstract
OBJECTIVE: To compare the combined antimicrobial effect of Thuja orientalis
and ciprofloxacin against Pseudomonas aeruginosa in vitro samples.
METHODS: In-vitro antibacterial activity of plant-extracts was evaluated alone
and in combination with ciprofloxacin against Pseudomonas aeruginosa using
disc-diffusion susceptibility assay (Kirby Bauer method) and minimum inhibitory
concentration (96 well broth microdilution method) following CLSI guidelines.
Pseudomonas aeruginosa clinical strains were collected from Rehman Medical
Institute (RMI) and Northwest General Hospital Peshawar, Pakistan and ATCC
strains (no.9721) of this bacterium were collected from Agriculture University
Peshawar. The organism was tested six times with crude extract and
fractionation with different solvents such as n-hexane, chloroform, ethyl
acetate and butanol at concentrations of 1, 4, 8, 12, 16, 20, 24, 30 and 36 mg/ml.
The mean MIC and FICI (fractional inhibitory concentration index) was
obtained to report the synergism. The data were analysed using SPSS version-
21.
RESULTS: In combination, methanolic crude extract, chloroform and butanol
fraction showed synergistic effect at all tested concentrations against
Pseudomonas aeruginosa (ATCC 9721 and clinical) except with 1, 4, 16 mg/ml
concentrations. Ethyl acetate and aqueous fractions shows indifference and
synergistic effect against Pseudomonas aeruginosa (ATCC 9721 and clinical) at
different concentrations. The fractional inhibitory concentration index (FICI)
ranged from 1.24 to 3.24 against Pseudomonas aeruginosa alone and in
combination with ciprofloxacin.
CONCLUSION: By disc diffusion method, this study shows synergistic effect
against Pseudomonas aeruginosa in combination with ciprofloxacin. However,
through minimum inhibitory concentration method, it shows antagonism and
indifference but no synergistic effect against different fractions of plant.
Hajra Mateen, Shafiq Ahmad Tariq, Syed Hamid Habib, Syeda Huma Khizar, Abdul Jalil Popalza, Abid Hussain. (2019) ANTIMICROBIAL ACTIVITY OF THUJA ORIENTALIS IN COMBINATION WITH CIPROFLOXACIN AGAINST PSEUDOMONAS AERUGINOSA, KHYBER MEDICAL UNIVERSITY JOURNAL, Volume 11, Issue 2.
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