Abstract
OBJECTIVE: To investigate the anti-oxidative and anti-nephrotoxic effects of
resveratrol in cisplatin induced nephrotoxic albino Wistar rats.
METHODS: This quasi-experimental study was performed at Isra University,
Hyderabad, Pakistan. Thirty male albino Wistar rats were divided into three
g roups: g roup-A ( control), g roup-B ( c ispl a tin) and g roup-C
(cisplatin+resveratrol). Biochemical [serum urea, creatinine and glutathione
peroxidase (GPX)] and renal histomorphology was performed in all groups
after 21 days of treatment.
RESULTS: Difference in mean pre- and post-experimental body weight was
observed in all three groups. Mean body weight decreased from 241.7±8.5 gm
to 196.50±9.34 gm and from 237±7.4 gm to 207.2±6.56 gm in group-B and
group-C respectively. In group-A; mean serum urea was 22.7±2.66 mg/dl,
serum creatinine was 0.45±0.05 mg/dl and serum GPX was 1.44±0.13 ηg/ml.
In group-B; mean serum urea level was 51±3.65 mg/dl, mean serum creatinine
was 0.78±0.05 mg/dl and serum GPX was 0.85±0.11 ηg/ml. In group-C, mean
serum urea level was 32.8±1.45 mg/dl, serum creatinine level was 0.41±0.09
mg/dl and serum GPX was 1.53±0.08 ηg/ml. In group-A, renal structure was
intact, marked changes were observed in renal histology of group-B while
group-C displayed less glomerular damage. The mean distance between
visceral and parietal layers of Bowman's capsule was 69.34±0.87 µm in groupA, 216.5±1.32 µm in group-B while 102.22±1.65 µm in group-C. Areas of
peritubular fibrosis and congestion were observed in groups B and C but less
prominent in group-C compared with group-B.
CONCLUSION: Resveratrol therapy is a potent anti-nephrotoxic regime
showing promising results in chemotherapy induced nephrotoxicity and
oxidative stress.
