Abstract
The present study was conducted to isolate and characterize pathogenic bacteria from eye infections.
The samples were taken from corneal eye infections. The collection of samples was done from “Layton Rehmat
Eye Hospital, Township Lahore”. Isolated bacterial pathogens were identified on the basis of morphological
and various biochemical tests. Morphologcal tests included different staining procedures includng Gram’s
staining and Endospore staining. Identification of pathogenic bacterial isolates was confirmed by growing
on selective medium. Antibacterial activity of different compounds was observed against the pathogenic
bacterial isolates. Minimum Inhibitory Concentration (MIC) test of different kinds of honey was performed
against the isolated bacterial pathogens. The antibacterial activity of leaf extract of Azadirachta indica (Neem
Plant) was also checked against the pathogenic bacterial isolates. The bacterial pathogens isolated from eye
infections were identified as Staphylococcus sp., Streptococcus sp., Neisseria sp., and Bacillus sp. These bacteria
commonly caused corneal infections. All the pathogenic bacterial isolates showed sensitivity against all the
compounds except Streptococcus sp. (BS1) and it showed resistance only against 3-Nitro-aniline (SAHC11). Bacterial isolate Staphylococcus sp. (BS4c) showed maximum sensitivity against methyl-amine (SAH5) with zone of inhibition of about 16mm in diameter while the bacterial isolate Staphylococcus sp. (BS6)
showed minimum sensitivity against Propyl-diamine (SAH-16) and Ethylamine cinnamaldehyde derivative
(SAHC-7) compounds with 1mm diameter of zone of inhibition under optimum growth conditions i.e.,
optimum pH and temperature. Antibacterial activity of leaf extract of Azadirachta indica (Neem Plant) was
also checked. Methanol is used as a standard against which all the pathogenic bacterial isolates showed
resistance. The minimum sensitivity showed by Staphylococcus sp. (BS4C) with the zone of inhibition of about
2mm in diameter while the maximum sensitivity showed by Staphylococcus sp. (BS6) with zone of inhibition
of 8mm. MIC test of four different kinds of honey was also performed. Different honeys showed different
results. For all the isolated bacterial pathogens, Pak honey inhibited the growth at 1ml concentration, Local
honey at 3ml concentration, Marhaba’s honey at 4ml concentration, and Young’s honey inhibited the growth
at the concentration of 5ml.
Nazish Mazhar Ali, Saiqa Andleeb, Iram Liaqat , Asma Ashraf , Bushra Mazha r, Shaukat Ali. (2019) Biochemical Characterization of Bacterial Pathogens from Corneal Eye Infections and their Control via Chemotherapy, Phytotherapy and Apitherapy, Journal of Innovative Sciences, VOLUME 5, ISSUE 1.
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