Abstract
Non-enzymatic glycation is a major source of irreversible AGEs
production with oxidative stress and can cause major diabetic
complications such as neuropathy, nephropathy, and cataract by
changing the structure and function of proteins. Common medicinal food
having antiglycation and antioxidant activity can be a good therapeutic
agent for the treatment of diabetes. The present study was undertaken to
investigate the antiglycation and antioxidant activity and their correlation
among extracts of four household condiments namely Allium sativum,
Zingiber officinale, Caralluma fimbriata and Momordica charantia. In
vitro Bovine Serum Albumin assay (BSA) was used for measurement of
antiglycation activity with four different solvent extracts (acetone,
methanol, ethanol and distilled water). 1-diphenyl-2-picrylhydrazyl
(DPPH) assay was used for detection of antioxidant activity. Methanolic
and acetone extract of all condiments showed significant antiglycation
and antioxidant activity and strong correlation (r=0.98). M. charantia
showed the highest antiglycation activity with acetone extract as well as
free radical scavenging activity 89.80%±1.37 and 93.43%±0.44 at 2mg/ml
concentrations on the 7th day of incubation, respectively. All the other
plants have shown their maximum percentage inhibition of glycation and
antioxidation in methanol and acetone extract. Hence, it is suggested that
these plants have a synergetic effect in many complications of diabetes
WAIS ASIF , ANAM SHEHZADI, NAEEM MEHMOOD ASHRAF, ROBINA RASHID, SAJID MEHMOOD. (2019) In Vitro Antioxidant and Antiglycation properties of extracts from Zingiber officinale, Allium sativum, Caralluma fimbriata, and Momordica charantia by using Nanofluorometry, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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