Abstract
In current work, effects of different drying procedures as well as of
different solvents on extraction of antioxidants from M. nigra leaves
have been studied. Initially, samples were dried using different drying
procedures (shade, oven and microwave drying) and extracted using
different solvents (ethyl acetate, acetone and methanol) in combination
with microwave-assisted extraction technique. Antioxidant potentials of
extracts were determined in terms of total phenolics, total flavonoids
and ascorbic acid contents. In addition, radicals (DPPH•
& ABTS•+)
scavenging potentials were also determined. Results revealed that
drying methods have no significant influence on recovery of
antioxidants from sample matrix. However, methanolic extracts
exhibited highest antioxidant potential that confirmed superiority of
methanol as extraction media over the other solvents used. In addition,
microwave-assisted extraction technique for the recovery of
antioxidants from M. nigra leaves was optimized using response
surface methodology. For the purpose, oven dried samples were
further extracted using methanol under different sets of extraction
conditions (microwave intensity and extraction time). A central
composite design was employed to determine the optimum conditions
for the maximum recovery of antioxidant components from leaf matrix.
Significant effects of different extraction conditions were recorded on
recovery of antioxidant components and radical scavenging potential.
On the basis of results, optimum conditions for the recovery of
antioxidant components from M. nigra leave have been prescribed.
UMER YOUNAS, SHAHID IQBAL , JAMSHED AKBAR. (2019) Optimization of extraction process for the recovery of antioxidants from Morus nigra leaves, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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