Abstract
This research aimed at assessing the antimicrobial properties of
Dalbergia, Brassica and Trifolium honey samples against
microorganisms isolated from infected burned skin of patients in children
hospital, Lahore, Pakistan. The isolated microorganisms were identified
as P. aeruginosa, E.coli, K. pneumoniae and S. aureus. The original
bacterial inoculum was serially diluted (adjusted to 1.5 X 106 CFU) and
spread on the nutrient agar plates. Whattmann filter paper discs were
soaked in three different concentrations (50%, 70% & 90%) of each of
the three unifloral honey samples for 48 hrs. The filter discs were placed
on the agar plates seeded with the individual bacteria. Solitary effect of
antibiotic discs (Ciprofloxacin, Imipenem, Ceftriaxone, Amikacin, and
Vancomycin) and their synergistic effect were also studied. No bacterial
growth showed resistance to honey at any concentration when used
individually or in combination with antibiotic although bacteria showed
resistance to Ciprofloxacin and Ceftriaxone. Brassica honey 90% was the
most effective at all concentrations with a maximum of inhibition zone
11.13 mm against P. aeruginosa followed by 90% Trifolium honey with
the maximum inhibition zone 10.75 mm against P. aeruginosa. The
Dalbergia honey (50%) was least effective against P. aeruginosa,
however it inhibited S. aureus producing inhibition zone 10.25 mm.
Honey-antibiotic combination produced inhibition zone 51 mm that was
much larger than the inhibition zone produced by antibiotic or honey
when applied individually. Honey, whether used individually or in
combination with an antibiotic, was effective against all the bacterial
isolates used in this study
IRFANA IQBAL, PAKEEZA TANWEER, FARKHANDA MANZOOR, MUHAMMAD NAUMAN AFTAB, AFSHAN KALEEM, ROHEENA ABDULLAH, ASMA ZAFAR, MEHWISH IQTEDAR. (2018) Antimicrobial properties of Dalbergia, Brassica and Trifolium honey against burn Microorganisms, Biologia – Journal of Biological Society of Pakistan, Volume 64 (II), Volume 64 (II).
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