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The current work describes the ultrasonicated assisted extraction followed by comprehensive investigation of antioxidant activity and αamylase inhibition potential of hydroethanolic leaf extracts of Vitexnegundo. Freeze drying assisted ultrasonicated extraction revealed highest level of total phenolic contents (258.29±2.52 mgGAE/g DE) and total flavonoid content (155.91±1.75 mgRE/g DE) in 60% hydroethanolic V. negundo leaf extract. Furthermore, total antioxidant power (254.35±1.56 mg AAE/g DE) and antidiabetic effectbased on α-amylase inhibitory assay (IC50=42.91±1.24 µg/mL) was also depicted to be highest in the same extract. Based on current findings, 60% ethanolic extract of V. negundoleaveswas revealed to be most potent for its antioxidant and α-amylase inhibition activity, hence could be used for formulation of new plant based functional food sornutraceuticals to improve human health.

MUHAMMAD NADEEM, MUHAMMAD WASEEM MUMTAZ , MUHAMMAD DANISH. (2019) Ultrasonication-assisted extraction, antioxidant activity and α-amylase inhibition potential of vitexnegundo leaves, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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