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Probiotics are used as a substitute for antibiotic growth promoters in the
chick industry. In the present study, conditions for the sporulation of
Bacillus subtilis, a probiotic strain, are optimized. Overnight grown
inoculum (1%) was used for the cultivation of B. subtilis in NB medium
followed by 24-48 hours incubation in a shaking incubator at 30°C. Spore
staining and microscopy were performed at different time intervals to find
out the sporulation status and time required for the completion of
sporulation. However, sporulation of the Bacillus strain completed after 22
hours and the total viable count (TVC) was found to be 8.3 x 1023 CFU/ml
(OD 1.872). Similarly, optimization of temperature (30°C) and pH (7) was
carried out that reduced the sporulation period up to 18 hours. The total
viable count was 6.4 x 1012 CFU/ml. The acid tolerance of the B. subtilis
was also determined at pH 2 and TVC after the acid treatment was 6.4 x
1012 CFU/ml. It depicts the ability of bacteria to withstand the acidic
conditions
IKRAM-UL-HAQ, MUHAMMAD SHRAFAT, UZMA HAMEED. (2019) Optimization of sporulation conditions of Bacillus sublilis as potential probiotics strain, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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