Abstract
Residual biopolymers in the distiller's grains (DGs) can be used as a protein source in animal husbandry. Aimed at this, nine
probiotics strains including Bacillus methylotrophicus, Bacillus subtilis, Bacillus megaterium, Acetobacterium balch,
Pediococcus acidilactici, Aspergillus oryzae, Candida utilis, Aspergillus carbonarius, and Mucor wutungkiao Fang were
screened for their fermenting DG in this study. The biotransformation effect of DGs was evaluated by the changes of crude
protein, soluble protein, content, and composition of 17 amino acids. Besides, 20% fermented DGs were added into the basic
mouse feed and the apparent digestibility of crude protein was detected. It was shown that the crude protein contents of DGs
products fermented by A. oryzae, B. subtilis and B. methylotrophicus fermented DGs were the highest, while the soluble protein
contents of DGs fermented by B. methylotrophicus, A. oryzae and A. balch were the highest. The amino acid contents of DGs
were increased by 33% after fermentation by B. subtilis. In the fermentation products of Bacillus spp. and A. oryzae, the
proportions of seven essential amino acids except for tryptophan in total amino acids were increased significantly with growth
rates of 10-13%. In the feeding experiment of Sprague Dawley rats, the apparent digestibility of crude protein in all groups
was increased, while the fermentation group of A. oryzae, B. methylotrophicus and B. subtilis were significantly higher than
that in the control group. It was demonstrated that the biotransformation effect of DGs in A. oryzae and Bacillus spp. groups
were the highest among nine probiotics, which could be very promising protein feed material. This study provided basic data
to support the idea of developing low-cost, efficient, and novel alternative protein-rich feed for the livestock.
Hui Zhu, Ning Wang, Muhammad Aamer Mehmood, Muhammad Kashif Saleemi, Yong Tang, Muhammad Qamar Bilal, Jun Xie, Baoxiang Gu , Huibo Luo. (2020) PROBIOTICS-BASED FERMENTATION ENHANCED THE PROTEIN CONTENT OF THE DISTILLER’S GRAIN FOR ITS APPLICATIONS IN ANIMAL HUSBANDRY, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 6.
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