Abstract
Olive leaves of eight varieties (Gemlik, Manzanilla, Sevillano, BARI-Zaitoon 1, BARI-Zaitoon 2, Earlik, Azerbaijan and
Hamdi) procured from Barani Research Institute were processed to obtain olive leaf extract. The extract was analyzed for
antioxidant capacity by measuring the concentration of oleuropein and rutin through HPLC and of total antioxidants through
ABTS, FRAP and DPPH assay. Antibacterial potential of the extract was calculated against Staphylococcus aureus,
Escherichia coli and Salmonella typhimurium through the methods of disc diffusion, minimum inhibitory concentration (MIC)
and minimum bactericidal concentration (MBC). The extract from the variety Gemlik, due to its highest antioxidant and
antimicrobial potential among the varieties, was used to prepare functional food packaging. The efficiency of Gemlik coated
packaging was measured by its thickness, water vapor transmission rate, oxygen transmission rate and antibacterial potential
through disc diffusion method. Olive leaf extract of Gemlik possessed the highest levels of oleuropein and rutin contents
(511.67±1.45mg/g and 6.98±0.01 mg/g of extract, respectively). Gemlik had the highest mean contents of FRAP, ABTS and
DPPH, always followed by Sevillano. Gemlik extract showed the highest zone of inhibition against E. coli (16.33±0.33 mm)
and against Salmonella typhimurium (16.00±0.00 mm), while Manzanilla had the highest value against Staphylococcus aureus
(15.00±0.00 mm). The mean MIC was significantly lowest for Manzanilla (1.30±0.13) against E. coli, for both Gemlik and
Manzanilla (0.78±0.00) against Salmonella typhimurium, for Hamdi(0.78±0.00) against Staphylococcus aureus (0.78±0.00).
The mean MBC was significantly lowest for both Manzanilla and Earlik (4.68±0.00) against E. coli, for Gemlik (1.56±0.00)
against Salmonella typhimurium, for Sevillano (1.95±0.00) against Staphylococcus aureus. The studies of thickness, water
vapor transmission rate, oxygen transmission rate and antibacterial potential of the packaging sheets proved olive leaf extract
an effective active functional food packaging. We recommend cultivation of Gemlik in Pakistan and animal studies to explore
health-promoting effects of locally grown olive cultivars
Fakhara Khanum, Tahir Zahoor, Muhammad Issa Khan, Muhammad Asghar, Shyam S. Sablani. (2020) ANTIOXIDANT, ANTIBACTERIAL AND FUNCTIONAL-FOOD-PACKAGING POTENTIAL OF LEAF EXTRACT FROM PAKISTANI OLIVE CULTIVARS, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 3.
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