Abstract
Pea (Pisum sativum L.) is an important edible leguminous seed crop. In the current study, salt stress was induced in Pea and
the expression pattern of Mn-superoxide dismutase, Peroxiredoxin, Thioredoxin, and Alternative oxidase genes was studied.
The research was performed in hydroponic system, with three replicates. Ten days old seedlings of Pea variety “Meteor” were
exposed to four different levels of salt stress i.e., 0 mM, 50 mM, 75 mM and 100 mM NaCl. The expression of selected genes
was studied by reverse transcriptase polymerase chain reaction (RT-PCR). Results showed that shoot and root length, root and
shoot biomass were significantly reduced under salt stress as compared to the control Pea plantlets. Results showed7.77 cm
shoot length and 9.89 cm root length under 100 mM NaCl as compared to 22.45 cm shoot length and 24.53 cm root length in
control. Fresh shoot biomass of 0.81 g and fresh root biomass of 2.3 g was observed under 100 mM salt stress as compared to
1.5 g fresh shoot biomass and 1.2 g fresh root biomass in control plantlets. Mn-SOD gene showed expression in all treatments
but high expression was observed under 75 mM NaCl and 100 mM NaCl treatments. Trxo1 gene showed expression in all
treatments but was more pronounced in 75 mM NaCl. The PrxIIF gene also showed the expression in all salt treatments and
the expression increased with increasing salt stress. AOX gene showed high expression in 50 mM and 100 mM NaCl and was
less expressed in 75 mM NaCl. As the expression of selected genes was upregulated in Pea leaves under salinity, therefore, the
results suggest that these genes are involved in salt stress and these may help to cope salt stress and could be used in developing
salt resistant Pea varieties in future
Sobia Manzoor, Rafiq Ahmad, Muhammad Shahzad, Muhammad Sajjad, Nadeem Khan, Umer Rashid, Amber Afroz, Sabaz Ali Khan. (2020) SALT STRESS REDUCES THE PEA GROWTH AND INDUCESTHE EXPRESSION OF SELECTED ANTIOXIDANT GENES, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 2.
-
Views
398 -
Downloads
46
Article Details
Volume
Issue
Type
Language
