Abstract
Drought stress is the major threat to maize development and production. Here the bulked segregant transcriptome analysis
(BSTA) was applied to discover the molecular mechanisms of drought stress responses in maize. The evaluation of drought
stress tolerance was contacted for 200 maize inbred lines. Two bulk pools were constructed by selecting 10 inbred lines that
were tolerant to drought (TD) and other 10that were sensitive to drought (SD). BSTA was performed to identify the
differentially expressed genes (DEGs) within two kinds of maize under drought treatment. A total of 4886 and 5274 DEGs
were detected in TD and SD bulk pools, respectively. The functional annotations showed that most of the DEGs are involved
in the hormone metabolism, transcription regulation and alternative splicing pathways. Comparisons of the DEGs between two
bulk pools revealed that the number of up-regulated genes related to alternative splicing in TD bulk pool was significantly
higher than that in SD bulk pool. Besides, 928 up-regulated genes and 346 down-regulated genes among 1274 DEGs were
identified including protein kinase andtranscription factors only in TD bulk pool. This result indicates that many common
mechanisms are involved in drought regulation, moreover alternative splicing may play dominant role in response to drought
stress in maize. Our results contribute to understand the mechanisms of drought stress tolerance of maize.
Kaiming Zhou, Xi Zeng, Binglin Zhang, Huawen Zou, Muhammad Aslam , Haiping Xin, Weijuan Liu. (2020) BULK SEGREGANT TRANSCRIPTOME ANALYSIS BASED DIFFERENTIAL EXPRESSION OF DROUGHT RESPONSE GENES IN MAIZE, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 4.
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