Abstract
In this study, resistance inheritance and cross resistance to Cry1Ac toxin and the insecticide triazophos in the pink bollworm
(Pectinophora gossypiella) was investigated. F1 progeny were simultaneously backcrossed with susceptible parents to obtainF2
progenywithless genetic resistance against the Cry1Ac toxin and insecticide triazophos. In two sets of experiments,
Cry1Ac/triazophos-resistant pink bollworms (Cry1Ac-R/Triazo-R)were selected through six generations and were reciprocally
crossed with susceptible laboratory strains (Cry1Ac-S/Triazo-S). Resistance ratios for reciprocal crosses of Cry1Ac and
triazophos were 16.25 and 27.11, respectively, while Resistant Parent RR values showing cross resistancewere10.51 and 6.50
folds, respectively. F1 progeny were again back-crossed with susceptible parents to prove the phenomena of refuge strategy
instantaneously reducing the resistance developeding pink bollworms. Parents, F1 and F2progenies were tested for their LC50
values. Results of cross resistance forCry1Acshowed, dominance value of resistance was below 1, proving that resistance was
completely recessive but autosomal in nature and was controlled by more than one gene. For triazophos, dominance was also
autosomal but was dependent on concentration, i.e., at low concentration it was incompletely dominant, at middle
concentration, partially recessive; while at higher concentration it was completely recessive. These findings are helpful for
pink bollworm control in Bt cotton expressing Cry1Ac combined with insecticides spray
Zunnu Raen Akhtar, Mansoor-ul-Hasan, Amer Rasul, Muhammad Sagheer , Bushra Saddiq, Irfan Ashraf , A sad Ali . (2020) CHARACTERIZING THE MODE OF RESISTANCE INHERITANCEAND CROSS RESISTANCE IN PINK BOLLWORMAGAINST CRY1AC TOXIN AND ORGANOPHOSPHATE PESTICIDES IN PAKISTAN, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 4.
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