Abstract
Herbal medicines have always been in practice since ancient times due to their ease and efficiency. Therefore, methanolic,
acetonic, chloroform and n-hexane extract from the seeds of Centratherum anthelminticum were prepared by soxhlet extraction
method. These extracts were subjected to quantify antioxidant abilities like TPC, TFC, DPPH free radical scavenging, reducing
power, radical scavenging capacity for hydroxyl and nitric oxide. The toxicity of the extract was assessed by hemolytic assay
in vitro on human and poultry erythrocyte. However, the percentage clot lysis behaviour of these extracts was also analyzed
by thrombolytic activity by using Clopidogrel as standard. Further, Antiviral effect was inspected by in ovo analysis against
Newcastle disease virus (NDV). Seven groups of nine-day-old embryonic chicken eggs were inoculated with different
concentrations of extracts. Except for the negative control group; all groups were inoculated with NDV strain and different
concentrations of four extracts. Observed the embryo's survival every day. Hemagglutination was done by collecting allantoic
fluid from experimental eggs. Results indicated that all the extracts have antioxidant potential and display the highest
antiradical behaviour in the pattern of methanolic > acetonic > chloroform > n-hexane. The hemolytic activity showed that all
extracts of C. anthelminticum are non/less toxic towards Human and poultry RBCs (< 15%). The amount of clot lysis ranged
from 19 to 51% compared to 65% and 6.4%, respectively provide by positive control and negative control. In the case of the
methanol extract, the level of clot lysis was the most abundant (51%). In the case of in ovo antiviral assay, the highest survival
rate was observed in the methanolic extract at 400µg/mL and acetonic extract at 300µg/mL as they control the NDV activity
ultimately, evidenced by the absence of embryo death and HA titre. Chloroform and n-hexane could not inhibit the virus
completely.
Syed Aftab Wajid, Rahat Andleeb , Asma Ashraf, Bisma Islam, Farkhanda Asad, Muhammad Umar Ijaz, Tayyaba Sultana. (2020) IN VITRO ANTIOXIDANT, HEMOLYTIC, THROMBOLYTIC POTENCIES OF Centratherum anthelminticum SEED EXTRACTSAND IT’S IN OVO ANTIVIRAL EFFICACY, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 5.
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