Abstract
The study aimed to document the therapeutic effect of application of Electroacupuncture (EA), oral administration of Nigella
sativa (NS), Eugenia caryophyllata (EU) oils and surgical resection of ileum on TNBS induced ileitis with visceral
hypersensitivity (VH) in dogs.I ml solution containing 30 mg of TNBS in 30 % ethanol was injected into ileal wall of dogs of
TNBS group, TNBS+ EA group, TNBS + NS group and TNBS + EU group. In saline group, an equal quantity of saline was
injected instead of TNBS solution. Dogs in the EA group were treated with EA at day 6, 9, 12, 15, 18 and 21 after TNBS
treatment while dogs in TNBS+ NS and TNBS+ EU group were orally administered with NS oil and EU oil @2.5 ml /kg on
day 6, 9, 12, 15, 18 and 21. VH in dogs of all groups was reflected by Pain Response Score (PRS).PRS was assessed by
monitoring Behavioral Pain Responses to Colorectal Distension at day 6, 9, 12, 15, 18 and21 in all groups.Blood samples were
taken from each group of dogs on day 6, 9, 15, 18 and 21 after colorectal distension test to measure the Plasma IL-6
Concentrations. Dogs were passed through laparotomy procedure to get ileal tissue samples from each group. Macroscopic
and microscopic scoring of each sample was done. Three dogs from saline group (n=3) and three dogs from TNBS group (n=3)
were further tested to see the effect of surgical resection of ileum on VH. After induction of ileitis with TNBS/Ethanol dogs
exhibited the signs of Vomiting, diarrhea, and decreased body weight. Macroscopic and Microscopic scoring of ileal tissue
was increased, plasma concentration of IL-6 was increased and Pain response score in response to colorectal distension was
also increased. EA application, oral administration of NSand EU oil relieved the VH by reducing the Pain behavior response,
Microscopic and Macroscopic scoring and plasma concentration of IL-6. Surgical resection of effected part of ileum also relieved
the VH.
Sayyed Aun Muhammad, Abdul Shakoor, Muhammad Ijaz, Syed Ehtisham-ul-Haque, Adnan Hassan Tahir. (2021) ATTENUATION OF VISCERAL HYPERSENSITIVITY DUE TO TNBSINDUCED ILEITIS BY ELECTROACUPUNCTURE, Nigella sativa, Eugenia caryophyllata OIL AND SURGICAL RESECTION OF ILEUM IN DOGS, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 1.
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