Abstract
Seeds and vegetative parts of two white and four pink flesh grapefruit (Citrus paradisi Macf.) varieties were exposed to colchicine treatment under in vitro and field conditions for the development of colchiploid germplasm for breeding programs. Genotype dependent plant growth responses were observed under both in vitro and field conditions. Embryo germination and plant growth were arrested at higher levels of colchicine. Cultivar Shamber had higher embryo germination (65.47%), number of leaves were more in Shamber and Red Blush (7.19) whereas long shoots and roots were developed in Red Blush and Red Mexican (4.07 cm and 9.18 cm, respectively). Under field conditions, Red Blush developed more axillary sprouts (2.78) and large sized leaves in colchicine treated branches. Shoot growth was more in Reed and Red Mexican (11.57 cm) while number of leaves were more in Shamber (8.79). Number of leaves were reduced from 12 (control) to 4 leaves at higher levels of colchicine (0.3%) under in vitro and field conditions. Stomata were maximum in Frost Fresh and Red Mexican (9.02) and their size was larger in Red Blush. Stomatal frequency was reduced (from 13.65 to 5.79) while size increased at higher levels of colchicine. These findings indicate existence of a strong genotypic variation in plant growth parameters and stomatal attributes in colchiploid germplasm. Further screening of the putative polyploids is suggested for the assessment of colchicine induced genetic diversity.
Muhammad Usman, Bilquees Fatima, Muhammad Usman, Iqrar Ahmad Rana. (2021) MORPHOLOGICAL AND STOMATAL DIVERSITY IN COLCHIPLOID GERMPLASM OF GRAPEFRUIT, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 2.
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