Abstract
American bollworm (Helicoverpa armigera) is poly-phagous pest which infects major fiber, pulses and vegetable crops, e.g.,
cotton, soybean, chickpea, brinjal, tomato, peas, beans, okra, and chili in Pakistan. Farmers use the different dosage of
insecticides to control American bollworm on various crops that might be due to the existence of genetically diverse American
bollworm population. So, it is essential to explore and get an understanding of this genetic variation existing among American
bollworm, feeding on different host plants. The current study was conducted to observe the genetic variation existing among
seven American bollworm populations by RAPD-PCR system collected from host plant including cotton, pea, green bean,
chili, green chili, tomato and okra. Overall, 210-bands were obtained from thirty RAPD primers, out of these, 34-bands were
polymorphic, and 176-bands were monomorphic. PIC values ranged from 0.2149 to 0.5014, with a mean of 0.2838. Genetic
similarity ranged from 92.38% (produced by cotton and peas) to 99.52% (produced by chili and green beans) and the genetic
distance range from 0.48% to 7.92%. A most important result was the clustering of the population occurring on green bean,
chili, green chili, tomato and okra in one cluster. In contrast, the population occurring on cotton and pea were designated
separate clusters, respectively. These results showed that population occurring on cotton and pea were most dissimilar to other
populations indicating that populations are closely related to each other. There might be an interspecific crossing frequency
among these populations.
Keywords: American bollworm, genetic diversity, host plants, insects, molecular markers, RAPD.
Ali Raza, Muhammad Atif, Ammad Ramzan1. (2022) Prevalence of variation among American bollworm (Helicoverpa armigera) collected from different host plant in Punjab-Pakistan, Pakistan Journal of Agricultural Sciences, Volume 59, Issue 2.
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