Abstract
In recent years, the demand for citric acid production has been increased due to its application in various advanced
medical fields, which can only be fulfilled by biotechnological fermentation using agro-industrial wastes. It is a
bidirectional beneficial process for cost effective manufacturing of citric acid and management of agro-industrial
waste products. This project was carried out in order to evaluate the enhanced production of citric acid by
Aspergillus niger using agro-industrial wastes. In the first step of study the liquid state fermentation (LSF) and solid
state fermentation (SSF) by Aspergillus niger was compared using molasses based medium and the different carrier
substrate. In a second set of study wheat bran was utilized for citric acid production via fermentation parameters was
optimized. The results showed significant (P≤ 0.05) difference among LSF and SSF and between different
substrates in LSF and SSF. Wheat bran showed significantly increased (P≤ 0.05) production of citric acid (P= 0.001)
compared to other carrier substrates used through SSF. It was observed that 4.5 pH, 60 % moisture content, 5ml/5g
o
inoculum size and 30 C temperature, are the initial requirements for maximum production of citric acid. It was also
observed that Yeast extract in 1% concentration was the best nitrogen source. Surfactants like Tween 80 and sodium
dodecyl sulphat (SDS) showed no effect on the citric acid.
Irum Javed , Muhammad Asgher , Sadia Noreen, Humara Naz Majeed, Tanzila Sahar. (2018) SOLID STATE FERMENTATION OF ASPERGILLUS NIGER FOR CITRIC ACID PRODUCTION USING AGRICULTURE RESIDUE AS SUBSTRATE, Journal of Innovative Sciences, VOLUME 4, ISSUE 1.
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