Abstract
Keratinase (E.C 3.4.99.11) is an extracellular protease having efficient
ability to degrade keratin found in feathers and hair. It has various
applications including leather, textile, pharmaceutical, detergent and
animal feed industries. In current study, keratinolytic bacteria were
isolated from the soil of poultry farm and different cultural conditions were
optimized to obtain maximum enzyme yield. The isolates were initially
screened by formation of zone of clearance on the skimmed milk agar
and then evaluated for keratinase production by using raw feathers as
substrate in submerged fermentation. Two isolates, showing maximum
enzyme production, were identified as Bacillus species on their
morphological and biochemical basis. Crude enzyme revealed that
Bacillus sp. IIB-B5 produced maximum keratinase (28.13 U/mL) at pH
7.0, at 40oC after 72 h incubation. While Bacillus sp. IIB-B9 showed
maximum enzyme production (26.59 U/mL) at pH 8.0, after an incubation
period of 96 h at 50oC. This study showed that these isolates have the
potential to be used in keratin hydrolysis
MARIAM AHMED, HAMID MUKHTAR, YESRA ARSHAD. (2019) Isolation and screening of keratinase producing bacteria from soil, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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