Abstract
Proteolytic enzymes are characteristically produced by Bacillus species
during sporulation and germination. The hydrolysis of spore proteins to
free amino acids, is accomplished by proteases, in first 20 minutes of
spore germination. The analysis of Spo mutants (strains which lack
Sporulation (Spo) gene or have its inactive form) revealed that many of
these strains did not produce extracellular proteases. Some evidence for
the involvement of serine proteases in sporulation has been provided by
inhibitor studies. Nevertheless variation in germination response of
germination defective strains with different nutrients was recorded in ALA
system, but overall responses of germination defective spores with
substituted amino acids and alanine are not much different. Therefore all
the amino acid proved competent germinant in place of alanine in ALA
system. The overall role of germinants as a replacement in AGFK is
satisfactory and they trigger germination in germination defective
bacterial spores as do asparagines. In present studies all the germination
defective spores showed very low proteolytic activity in comparison with
proteolytic activity of normal spores of wild type Bacillus subtilis PY79. It
appears that defective spores lack enzymes with proteolytic activity which
is necessary for germination. Since all protease deficient mutants are
asporogenous. Although some mutants, producing very low levels of
protease are still able to sporulate, with some drawbacks.
Uqba Mehmood . (2019) Proteolytic activity analysis in germination defective spores of Bacillus, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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