Abstract
Tumor necrosis factor alpha is known as multifunctional cytokine component
of apoptosis and cell survival. Preterm delivery is a confused prenatal
unfavorable occasion. This examination work was planned to associate
relationship of G308A and -238 polymorphisms in Tumor necrosis factor
alpha. We collected blood samples of 70 patients out of which 50 ladies gave
untimely births and 20 ladies gave typical births. DNA extraction technique
was applied. Primers were designed, amplified by PCR and RFLP were
performed to identify the TNF–alpha -308 and -238 polymorphisms. The
frequencies of TNF-alpha -308 genotype TNF GG, GA and AA were 37%,
10% and 3% with measurably estimation of 0.07039 (P<0.05) as indicated by
HWE for the preterm and postterm conveyances while for the typical one
proportion of GG, GA and AA were of 5%,10% and 5% with factually critical
esteem is 1.000 (p<0.05). Conclusion: If there should happen an occurrence
of TNF-alpha -238 genotype of GG, GA and AA were 35%, 12% and 7% of
those ladies who conveyed untimely births and in typical ones factually critical
esteem was additionally more than 0.05. The significance level of 0.005872 of
TNF-alpha -308 and 0.010089 in TNF-alpha -238 as indicated by Exact
Fisher test are important. However, further study on large sample size would
increase the risk of pre and post term births in Lahore.
UZMA PARVEEN, MASROOR ELLAHI BABAR, NIDA BABAR, TAHIRA MUGHAL, AKHTAR ALI. (2019) Genetic evaluation of tumor necrosis factor-alpha gene polymorphisms with pre and post term births, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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