Abstract
Brassicaceae member i.e. Rhaphinus sativus L. and three species of Trichoderma (T. harzianum, T. viride and T. hamatum) were evaluated for their antifungal effect against Sclerotium rolfsii, the causal agent of collar rot disease in chickpea. In vitro, methanolic leaves extract of R. sativus significantly decreased pathogen biomass by 6-96% and three Trichoderma species also caused significant inhibition in pathogen growth variably between 30-100%. In potted soil, application of separate or combined application of myco- and phyto-fungicides proved highly efficient in managing collar rot disease. However, combined effect of T. harzianum or T. viride + leaves biomass of R. sativus found more promising in managing disease through significantly improving growth attributes and activities of antioxidant enzymes. It was concluded that disease suppression is directly linked with increase in activities of antioxidant enzyme (peroxidase, catalase, polyphenol oxidase and phenylalanine ammonia lyase) thus provides the basis for resistant in chickpea.

AMNA SHOAIB, AROOJ SHEZAD, ARSHAD JAVAID, SUNDUS AKHTAR , ZOIA ARSHAD AWAN. (2019) Evaluation of biocontrol strategies and its synergistic interaction permitting the chickpea plant to trigger the appropriate defense responses against Sclerotium rolfsii, Biologia – Journal of Biological Society of Pakistan, Volume 65 (II), Issue 2.
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