Abstract
Tetrastigma hemsleyanum is a precious and rare traditional Chinese medicinal plant. Flavonoid is its main medicinal ingredient.
Wild T. hemsleyanum (W-TH) growing in Zhejiang Province, China is recognized as a medicinal material of “San Ye Qing”
Dao-di herbs. The different origins and thus the contents of medicinal ingredients are the key criteria used to determine whether
the medicinal materials are authentic Dao-di herbs. However, it is less known how the eco-environments of its specific
producing area, especially microbial community in rhizosphere soil, affect the content of medicinal ingredients in “San Ye
Qing”. In the present study, we determined the content of total flavonoids and the enzymatic activity of phenylalanine
ammonia-lyase (PAL) in the roots of W-TH and artificially cultivated T. hemsleyanum (C-TH), as well as the nutrient
compositions and metagenome in rhizosphere soil. The effects of the rhizosphere soil on the flavonoid metabolism in T.
hemsleyanum were evaluated. The contents of total flavonoids and the PAL activity were higher in W-TH root than in C-TH
root. The contents of both available P and available K were higher in the rhizosphere soil of W-TH than in that of C-TH, while
the contents of N and organic matter in the rhizosphere root of C-TH were higher than that of W-TH. Compared with the
rhizosphere of C-TH, the abundances of genera Enterobacter, Serratia, Raoultella, Kluyvera, Comamonas, Acinetobacte, and
Arthrobacter, and the pathways related to N metabolism, inositol phosphate metabolism, phosphinate metabolism, and
phosphotransferase system in the rhizosphere of W-TH were significantly different. There existed differences in
phenylpropanoid biosynthesis and phenylalanine metabolism in the rhizospheres of W-TH and C-TH. The contents of N and
available P in the rhizosphere soil affect diversity abundance, N metabolism, Pmetabolism, and phenylalanine (Phe) anabolism
of rhizosphere microorganisms. They may further affect Phe content and PAL activity for the synthesis of flavonoids in the
root of T. hemsleyanum
Taihe Xiang, Xiaxiu Tong, Jiangshan Li, Zhengxian Xu, Xinyuan Zhou, Shuying Bao, Chongshun Li. (2020) EFFECT OF RHIZOSPHERE SOIL ON FLAVONOID METABOLISM IN ROOTS OF Tetrastigma hemsleyanum, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 3.
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