Abstract
Whitefly-transmissible begomoviruses (Family-Geminiviridae) are the major pathogens of economically important crops and
weeds. In 2004-05, during field surveys in Faisalabad, Pakistan, symptoms of leaf curling and leaf deformations were spotted
on leaves of Chenopodium album. Chenopodium album is also known as Bathu (local name). This plant is commonly found
in crop fields and around water channels. Fifteen plant leaves showing upward leaf curling were collected from different
farmer’s fields. Complete virus and associated satellite molecules were amplified through rolling circle amplification (RCA)
followed by restriction digestion with suitable enzyme, cloning and sequencing. Basic local alignment search tool (BLAST)
showed that leaf curling disease of C. album is associated with Pedilanthus leaf curl virus (PeLCV) along with two different
types of betasatellite molecules i.e., Digera yellow vein betasatelite (DiYVB), Cotton leaf curl Multan betasatellite (CLCuMB)
and one alphasatellite i.e., Ageratum enation alphasatellite (AEA). Sequence analysis showed that PeLCV is a new variant
showing 95-98% sequence homology to PeLCV isolated from India and Pakistan. Associated betasatellites i.e., DiYVB and
CLCuMB showed 96-99% sequence homology to satellites isolated from India and Pakistan. Ageratum enation alphasatellite
associated with PeLCV was also a new combination and showed 99% sequence homology with other isolates from Pakistan.
This is the first report of PeLCV and associated satellites infecting C. album. The data presented in this paper would help in
understanding the diversity and etiology of begomoviruses in C. album.
Sehrish Ijaz, Muhammad Mubin, Asif Ali Khan, M. Shah Nawaz-Ul-Rehman. (2020) MOLECULAR ANALYSIS OF PEDILANTHUS LEAF CURL VIRUS AND ASSOCIATED SATELLITES INFECTING Chenopodium album IN PAKISTAN, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 2.
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