Abstract
In present study efforts were made to elucidate the importance of oilseeds as non-conventional protein sources. Purposely,
three different oilseeds i.e. sesame, flaxseed and canola were initially subjected to proximate and mineral composition.
Afterwards, defatted oilseed meals were used to prepare protein isolates through isoelectric precipitation method. The resultant
protein isolates were examined for protein content, recovery and yield. Moreover, functional properties i.e. water and oil
absorption capacities, foaming and emulsifying properties along with nitrogen solubility index and least gelation concentration
of protein isolates were also determined. The results indicated highest crude protein content in whole as well as defatted sesame.
Likewise, sesame protein isolates (SPI) exhibited maximum values for crude protein content (90.14±2.37%) followed by
canola protein isolates CPI (89.75±3.58) and flaxseed protein isolates FPI (86.37±3.69). Likewise, protein isolates recovery
was maximum in SPI (36.86±1.22) trailed by FPI (31.59±0.98) and CPI (30.52±1.20). Similar trend was noticed for yield i.e.
SPI (79.03±2.18), CPI (78.53±4.02) and FPI (74.61±2.93). Moreover, higher water absorption capacity was revealed in SPI
followed by CPI and FPI. Similarly, maximum foaming capacity was observed in SPI tracked by FPI and CPI. Conclusively,
defatted oilseed meals contain appreciable quantities of quality proteins. In the nutshell, sesame protein isolates exhibit better
recovery, yield as well as functional properties in comparison with flaxseed and canola protein isolates. Therefore, these can
be a potential candidate for utilization in various food formulations
Muhammad Sibt-e-Abbas, Masooq Sadiq Butt, Muhammad Shahid, Moazzam Rafiq Khan, Muhammad Tauseef Sultan, Muhammad Suffyan Saddique. (2020) NUTRITIONAL AND FUNCTIONAL CHARACTERIZATION OF DEFATTED OILSEED PROTEIN ISOLATES, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 1.
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