Abstract
This is the first report of resistance development including the mode, inheritance of resistance, and life parameters in field
collected as well as lambda-cyhalothrin selected laboratory strains of Mythimna separata. Field collected and laboratory
selected strains were further assessed for cross-resistance with organophosphate insecticides: chlorpyrifos, triazophos,
profenophos, quinalphos, and metha-midophos. For field resistant and susceptible strains, the LC50 was determined for 2016
and 2017. In laboratory studies, lambda-cyhalothrin-resistant M. separata (Lambda-R) were selected for 14 generations and
reciprocally crossed; F1 progenies were simultaneously backcrossed with susceptible parents (Lambda-S) to examine the
phenomena of refuge strategy by instantaneously reducing the resistance developed in M. separata resulting in F2 progenies
with diluted resistance against lambda-cyhalothrin. Both field collected (G1) and laboratory selected populations (G14) (Parents,
F1 and F2 progenies) were tested for their LC50 values. RR ratio, through bioassay, was higher in the lambda-cyhalothrin
selected strain than the susceptible strain, which confirms resistance in M. separata to lambda-cyhalothrin. The h value
explicitly reveals that resistance is incomplete dominant and autosomal in nature and is controlled by more than one factor.
Reciprocal crosses of F1 progeny show cross resistance to other organophosphate insecticides, which shows that different
insecticides can control the insect. Cross resistance of the lambda-cyhalothrin selected strain further showed that the LC50 value
of organophosphate insecticides was closer to the field collected population of insects showing higher levels of cross resistance
of lambda-cyhalothrin and other organophosphate insecticides sprayed for oriental armyworm. These results are helpful to
manage outbreaks of oriental armyworm in maize and cotton fields.
Amer Rasul, Zunnu Raen Akhtar, Malik Abdul Rehman, Saif-Ur-Rehman, Tariq Mustafa, Asad Ali, Muhammad Sagheer, Habib ur Rehman, Muhammad Liaqat Mukhtar. (2021) FIELD EVOLVED RESISTANCE AND INHERITANCE PATTERNS OF LABORATORY SELECTED STRAINS OF ORIENTAL ARMYWORM, Mythimna separata, AGAINST LAMBDA-CYHALOTHRIN AND ITS CROSS RESISTANCE TO OTHER INSECTICIDES, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 1.
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