• Iqra Yousaf

• Shabab Nasir

• Syed Makhdoom Hussain

• Azhar Rafique

• Muhammad Umar Ijaz

• Muhammad Asrar

• Saif-Ur-Rehman

• Bilal Ahmad

• Mahid Iftikhar

• Naveed Ahmad Khan

Abstract

Pakistan being a tropical country, characterized by poor socioeconomic conditions, is more vulnerable to Mosquito-borne diseases. So, this study was proposed to know the prevalence of mosquito-borne diseases in Faisalabad district, Pakistan. For this, mosquito adults and larvae were collected from different habitats with aspirator and dippers respectively from eight towns of Faisalabad district. These samples were carried alive to laboratory and identified with the help of identification keys. Aedes female mosquitoes were killed and stored at -20°C for RNA extraction. Slides were prepared from blood of female Anopheles mosquitoes for the identification of plasmodium under microscope. The genome RNA from female Aedes mosquitoes were extracted as per commercially available kits according to manufacturer guidelines and stored at -40 0C for further use. Using online software, primers and probes for the known human pathogens were designed in consensus with the help of available genome database for different pathogens such as dengue virus. Plasmodium was detected from the mosquitoes that were mostly collected from rural areas and this was generally detected from the samples collected from Jaranwala and Samundri town. Dengue virus was detected generally from city areas and serotype 2 and 3 was detected from the Aedes mosquitoes. Dengue virus was mostly detected from Lyallpur, Madina and Jinnah towns. Mosquitoes were mostly collected after monsoon season in the months of August-September and in spring (March). The disease pathogens were also detected during these months. Through the surveillance of pathogens, we can focus our attention on the hot spots and apply control measures to control mosquitoes for better management.

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