Abstract
Antimicrobial resistance is a very serious threat to human health; hence the search for novel antimicrobials is urgently needed.
Cyclotides are unique disulfide rich mini-proteins of plant family having nearly 30 amino acids with N and C terminals joined
to induce cyclization. The exclusive structural cyclic cystine knot motifs (six conserved cysteine residues) make these stable
molecules against resistant antimicrobial strains. The Solanaceae family is phylogenetically fourth distinct family of plants
expressing conserved cyclotides. We investigated thirteen Solanaceae species displaying high antimicrobial activities against
different microbial strains for identification and isolation of cyclotide genes. The cyclotide encoding genes were found in four
Petunia x hybrida cultivars and in Solanum lycopersicum. The homology of the cyclotide encoding genes was compared with
homology sequence patterns of the already reported PETUNITIDE (Petunia x hybrida) genes. Phylogenetic analysis of novel
cyclotide precursor genes from P. x hybrida cultivars revealed that they had a close relationship with their homologues from
Solanaceae and formed three separate clades while S. lycopersicum showed a relationship with Fabaceae and formed a single
clade. Solanaceae precursors are distinct from other previously known plant family precursors. Novel cyclotide precursor
discovery within the Solanaceae plant species, defined their exclusive structural sequences, array of antimicrobial activities
and evolutionary link to their diversity from other families.
Zirwah Rizwan, Nosheen Aslam, Farheen Zafar, Rashida Humma, Amer Jamil. (2021) ISOLATION OF NOVEL CYCLOTIDE ENCODING GENES FROM SOME SOLANACEAE SPECIES AND EVOLUTIONARY LINK TO OTHER FAMILIES, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 1.
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