Abstract
Aflatoxin B1 (AFB1) is one of the toxic secondary metabolites of fungi belonging to the genus Aspergillus. AFB1 causes hepatotoxicity and carcinogenicity. Mostly, cereals, nuts, fruits, and stored commodities are prone to their attack. It has great health and economic importance. Under suitable conditions, fungus shows maximum pathogenicity which leads to the abundant production of AFB1. This study encompasses the selection of the most pathogenic strain producing abundant AFB1. For this purpose, 50 isolates were tested and YF18 was the most pathogenic. This isolate was then evaluated on 15 culture media which concluded that Yeast Extract Sucrose (YES) agar and Yeast Peptone Dextrose (YPD) agar media proved best for the optimal growth of A. flavus and abundant yield of AFB1. Moreover, the culture media showing optimal growth under different levels of growth conditions i.e., temperature, pH, and water activity, and their effect was verified with the aid of correlation and response surface methodology. The culture media were checked at five levels of temperature (15oC, 20oC, 25oC, 30oC, and 35oC), pH (3.5, 4.0, 4.5, 5.0 and 5.5), and water activity (0.80, 0.85, 0.90, 0.95, and 0.995) were tested, confirming that temperature of 30oC, pH of 5.5 and water activity level of 0.995 significantly enhanced the growth of A. flavus and production of AFB1 (P
Muhammad Humza. (2021) EVALUATION OF GROWTH MEDIA AND IN-VITRO CONDITIONS FOR THE ASSESSMENT OF Aspergillus flavus GROWTH AND AFLATOXIN B1 IN GRAPES, Pakistan Journal of Agricultural Sciences, Volume 58, Issue 2.
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