Abstract
Herbicides are chemicals that are widely and unconsciously used to control unwanted weeds. Herbicides do not only damage
the target animals, but they also damage the off-target soil fauna. In our study, it was aimed to determine the mutational
genotoxic consequences of commonly used Oxadiazon (OD) and Pendimethalin (PM) herbicides in agriculture fields on
Eisenia hortensis (E. hortensis) species by using Comet and Micronucleus tests. Consequently, the LD50values for OD and PM
herbicides were estimated at0.25 ppm and 0.24 ppm, respectively. Concentration series of OD and PM herbicides, ½ LD50,
LD50, and 2 × LD50 were applied to E. hortensis for 48 h. As a negative control group, distilled water was used for both
herbicides. An increase inthe concentration of damaged DNA and chromosomal aberration of E. hortensis coelomocytes was
observed by both herbicides. Highest DNA damage (32.33 ± 2.51; 15.33 ± 1.15) was observed by the highest concentrations
of OD (0.51 ppm) and PM (0.48 ppm), respectively. The negative control group had shown the least genotoxic effects. There
was statistically significant (p <0.05) difference among different concentration values of OD and PM herbicides.
Keywords: Genotoxicity, herbicides, micronuclei, comet assay, chromosomal aberration.
İbrahim Hakkı Ciğerci, Tuba Taşcan, Muhammad Muddassir Ali. (2022) Genotoxic assessment of oxadiazon and pendimethalin herbicides on Eisenia hortensis by comet and micronucleus tests, Pakistan Journal of Agricultural Sciences, Volume 59, Issue 2.
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