Abstract
Peas are growing all over the world as a leguminous crop due to high nutrients value. Fusarium wilt of peas is a destructive
disease and causing deleterious loses in pea growing regions of the world. The fields were surveyed with disease incidence of
Fusarium wilt in major pea growing areas. Fields with heavy pathogen infestation and natural disease suppressive were
observed at District Sahiwal, Pakistan. The samples were collected to diagnose the disease and factors responsible in the
suppression of disease. The results of soil physio-chemical properties showed no significant differences between diseased and
suppressive soils. Pathogenicity assays both in-vitro and pot trial showed that suppressive soil has natural ability to suppress
the disease. Furthermore, in-vitro and pot assays were designed with different soil treatments to investigate the factors
responsible in the natural disease suppressiveness in suppressive soil. The results demonstrated that the mechanism involved
in disease suppressive soil is biotic in nature. All isolated fungal strains from diseased and healthy roots of pea were subjected
to biological assays to evaluate the virulence. The assays showed that isolate SAH09 is non-pathogenic Fusarium oxysporum
which was isolated from the pea roots of suppressive soil. Isolate SAH09 was used in dual culturing technique and pot trial to
evaluate the mycoparastism behavior against virulent pathogenic isolates SAH03, SAH05 and SAH10. Results concluded that
isolate SAH09 of non-pathogenic Fusarium oxysporum has potential to suppress the growth of all isolates of pathogenic
Fusarium and possibly play the role in natural disease suppression in suppressive soils.
Keywords: Biological control, disease conducive soil, disease inhibition, incidence, nutrient competition.
Saman Aslam, M. Imran Hamid, Muzammil Hussain. (2022) Non-pathogenic Fusarium oxysporum contributes in the biological suppression of pea wilt in disease suppressive soil, Pakistan Journal of Agricultural Sciences, Volume 59, Issue 2.
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