Abstract
The objective of the current research was to assess the optimal extraction conditions with respect to ethanol concentration and
time to recover betalains from red beetroot (Beta vulgaris L.). Purposely, betalains were extracted from beetroot using water
and ethanol based solvent modules at three different time intervals i.e., 20, 40 and 60 min and the obtained extracts were
analyzed for betacyanins, betaxanthins and total betalains. The obtained data was further analyzed using three level-two factor
central composite design using response surface methodology. The data was fitted to second order polynomial equation by
applying multiple regression analysis. The optimal conditions were obtained using maximum desirability function that found
to be 31.03, 45.01 and 35.34% aqueous ethanol for betacyanins, betaxanthins and total betalains, respectively whilst, optimum
extraction time was recorded to be around 43 min. The experimental values were close to the predicted values that proves the
adequacy of the model employed. It was suggested that aqueous-ethanol is suitable for maximal recovery of beetroot betalains
instead of ethanol or water as a sole solvent.
Iahtisham-Ul-Haq, Masood Sadiq Butt, Muhammad Atif Randhawa, Muhammad Shahid. (2020) EXTRACTION, CHARACTERIZATION AND OPTIMIZATION OF BETALAINS FROM RED BEETROOT USING RESPONSE SURFACE METHODOLOGY, Pakistan Journal of Agricultural Sciences, Volume 57, Issue 2.
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